FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59968759
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG101750.014.112057120
CGTCGTG1564850.011.460366120
AGCGTCG1911200.011.338794120
GTCGTGT1442100.011.261014120
CGTGTAG1195900.011.142779120
TCGTGTA1293050.010.951437120
GCGTCGT1704500.010.775199120
GTGTAGG1233200.010.401386120
GAGCGTC2178700.010.349228120
AGAGCGT2362400.010.17009120
AAGAGCG2637600.010.163599120
GTAGGGA1088200.09.656238120
TGTAGGG1237700.09.242266120
TCGGAAG3707450.09.186667120
CGGAAGA3488600.09.1257715120
TAGGGAA1097600.09.048062120
GATCGGA4188950.08.830612120
ATCGGAA3919650.08.819611120
AGATCGG4533150.08.572288120
GAAGAGC3471800.08.174892120
Produced by FastQC (version 0.11.5)