FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57767514
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG378650.012.2956085120
AGTCACG740200.012.1905985120
CGTCTGA2664550.011.762661120
GTCACGT677600.011.607951120
ACGTCTG2953300.011.408953120
ACACGTC3574200.011.286912120
CACGTCT3279650.010.9487095120
CACACGT4007650.010.498816120
TCTGAAC2570000.010.383839120
TCACGTT684350.010.239785120
GAACTCC1910800.010.188864120
GCACACG4351600.010.169468120
GTCTGAA2793500.010.102874120
GTTTCGG416600.010.066631120
TGAACTC2174400.010.046331120
CTGAACT2451300.09.846453120
AGCACAC5026050.09.823053120
AACTCCA1916050.09.478331120
ACGTTTC563050.09.387614120
CACGTTT655750.09.3597355120
Produced by FastQC (version 0.11.5)