FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57767514
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG106350.032.10104120
CGTGTAG2865650.011.580434120
GATCGGG103200.011.569582120
AGAGCGG207500.011.363674120
GTGTAGG2733000.011.240215120
GTCGTGT3465300.011.133058120
CGGAAGG235050.011.103844120
CGTCGTG3786600.010.899842120
TAGGGAA2181000.010.764615120
TCGTGTA3117900.010.712809120
TGTAGGG2599350.010.590171120
GTAGGGA2269400.010.548875120
AGCGTCG4568050.010.541747120
TCGTGTG143850.010.343943120
AGTGTAG897750.010.32565120
GAGTGTA947250.010.223114120
CGCCGTA108000.010.055395120
GAGCGTC5042700.09.931425120
GCGTCGT4110800.09.809612118-119
GATCTCG447050.09.461877120
Produced by FastQC (version 0.11.5)