FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22906755
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG3754921.6392195228001523TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT602730.2631232577464595TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG471600.039.7558745
GATCGGA509100.037.245331
ATCGGAA514250.036.750622
TCGGAAG527600.035.799493
CTCGTAT538650.034.83767744
ACACGTC554150.034.57159813
CGGAAGA561750.034.3837854
ACGTCTG555600.034.33575415
TCTCGTA548600.034.25496743
ATCTCGT548600.034.20910342
GCACACG563450.034.148711
CACGTCT560500.034.12782714
AGTCAAC553250.034.06657434
CACACGT565400.033.96328712
CGTCTGA565200.033.764516
AATCTCG560400.033.59704241
AGAGCAC594200.032.589598
AACAATC579450.032.55434838
GTCAACA581050.032.4789935
GAGCACA598850.032.3740929
Produced by FastQC (version 0.11.5)