FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26635852
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG37550.028.22384843
CGTATGC39850.026.53805444
CTCGTAT41000.025.6840342
GATCGGA43450.025.1493991
ACACGTC52050.021.17781413
ATCGGAA54450.020.1440092
ACGTCTG57150.019.2092415
CACACGT58800.018.86146412
CACGTCT59250.018.79418414
TCGGAAG61500.018.273663
GTATGCC58950.018.20658345
TCTCGTA59800.017.79738841
CACTTAG63900.017.14659531
ATCTCGT63400.017.0707340
CATCTCG66700.016.4622639
CGCGTAA3850.016.3861
GCACACG70600.016.15507711
TAGGCAT67350.016.03566235
CGTCTGA76550.014.60565716
ACTTAGG77700.014.44888132
Produced by FastQC (version 0.11.5)