FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29382389
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG14250.021.47704343
CGCGTAT908.185507E-620.0217041
CTCGTAT15350.019.93797342
GATCGGA17300.017.7070561
TCTCGTA20200.016.0418441
GACGAAT29650.012.59644827
ATCGGAA24650.012.5949542
AATTACG13450.010.87528945
ATCTCGT30250.010.86074240
CGCGATT4457.2759576E-1210.62951
TATCCCG14550.010.20727939
CGGTAAT14900.010.1163292
GTTGTCG9150.09.6005721
CCGGTAA15750.09.5818151
GGACGAA40450.09.51135326
ACATCGG48650.09.43619845
CGAATGC40700.09.34332329
CTAAACG8800.09.2145341
CGGCAAT14000.09.1594193
GCGTCGA9450.09.0463411
Produced by FastQC (version 0.11.5)