FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50771969
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC562280.11074614813540125TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG138250.022.71995543
CTCGTAT146500.021.39459642
CGTATGC148450.021.08281544
AATCTCG156100.020.35251839
GTATGCC162200.019.40659745
TCTCGTA162950.019.33127241
ATCTCGT165600.019.11701640
GATCGGA156950.018.5143571
ATCGGAA169200.017.199842
ACACGTC194800.016.6763113
ACCAATC200100.016.19193636
ACGTCTG201000.016.13966215
GACCAAT201750.016.07026735
CACGTCT207900.015.77713814
CACACGT220850.014.73984412
TCGGAAG206300.014.2990783
CGTCTGA229550.014.12249516
GCACACG242300.013.86205911
ACTGACC255000.012.898931532
CCAATCT259800.012.59246537
Produced by FastQC (version 0.11.5)