FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57124602
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1751850.30667172088131134TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG267300.032.65088743
CGTATGC271600.032.0343244
CTCGTAT272200.031.95575142
TCTCGTA286300.030.41339541
GTATGCC290850.029.99923545
TATCTCG293450.029.47267739
ATCTCGT303950.028.73595440
CGCCAAT319950.026.99579433
GTCACGC343700.025.01830729
AGTCACG345750.024.86999128
ACGCCAA366350.023.89549332
ATATCTC389850.022.36368438
CAATATC397900.021.973436
CCAATAT404550.021.49522235
GCCAATA407500.021.33953734
ACACGTC419250.021.14269813
ACGTCTG427150.020.63085715
CACGTCT435150.020.37023414
CACGCCA446100.019.52757631
CACACGT461200.019.26837312
Produced by FastQC (version 0.11.5)