FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22471821
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC332610.1480120369417325TruSeq Adapter, Index 9 (100% over 51bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC241840.10761922676404373TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC69650.024.29645745
AGATCGG53050.023.6492481
TCGTATG74900.022.32308243
CTCGTAT76800.021.38990242
GATCGGA78050.021.2687571
TCTCGTA77800.021.11454641
ATCGGAA81350.020.548442
CGTATGC82600.020.3782944
ACACGTC84950.019.75608313
ACGATCA86450.019.39037732
AGTCACG86200.019.3680928
ATCTCGT86300.019.19100840
ACGTCTG88550.018.90208615
GTCACGA89050.018.87518929
TCACGAT89750.018.60274330
CGATCAG90000.018.6004533
CACGTCT92150.018.45660814
CACGATC91150.018.31648831
CACACGT96700.017.4718512
CGTCTGA99150.016.92670616
Produced by FastQC (version 0.11.5)