FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18157564
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGA15600.012.54632311
GTTACGA6850.011.823234
TCGTATG6300.011.7876743
TGTTACG6900.011.7375553
CGCGTAA7450.011.488891
TCGATTA8900.011.3748773
CGTTCGA14250.011.37027644
ACATCGG25650.011.142138545
TACGACT7300.011.0943396
ACGGACA23750.010.79896618
TATCGAC5250.010.7127897
TAAACGT7150.010.6983362
GCGTAAC8750.010.5419062
CGAAACG10600.010.4026444
AACGAGT11800.010.3077161
TAACGGC13600.010.25690727
CGATTAG10450.010.1182584
CCCGGAT31250.010.0908741
CGTCGAT19700.010.04935612
GTAACGG13950.09.99948426
Produced by FastQC (version 0.11.5)