FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20716024
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG265330.12807959674115071TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG38100.034.37706445
GATCGGA52700.025.946011
CTCGTAT49750.025.78413244
ATCGGAA55500.024.6050872
TCTCGTA52800.024.3800743
ACACGTC61950.021.96971513
AATCTCG59400.021.63224441
ATCTCGT60800.021.2832142
ACGTCTG66300.020.32467515
CACGTCT67800.019.97455814
TCGGAAG71300.019.4988653
CACACGT75150.018.29037312
CGTCTGA79050.017.160516
GTCACAT83050.016.60871329
GCACACG87900.016.25156411
ACATGTC87000.015.69896332
GAATCTC85200.015.42481240
AGATCGG31750.015.3960211
CGGAAGA93850.015.3650544
TCACATG94300.014.91363530
Produced by FastQC (version 0.11.5)