FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27889766
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG301880.10824042051840808TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG44100.035.2041145
CTCGTAT52700.029.41816344
TCTCGTA58050.026.70640443
GATCGGA57200.026.6300831
TATCTCG65200.024.26064141
ATCGGAA64050.023.8808692
AGTCACG66650.023.82980328
GTCACGT66800.023.70889329
ACACGTC68150.023.37065113
ACGTCTG70200.022.46385815
ATCTCGT69800.022.40399642
CACGTCT74150.021.6009214
TCACGTG79850.019.9468130
TCGGAAG80500.019.1405753
CACACGT85900.018.410512
ACGTGGC90600.017.82957332
GCACACG95550.017.39871411
GCCTTAT91800.017.20552637
CGTCTGA96000.016.7547316
GGCCTTA98650.016.17000836
Produced by FastQC (version 0.11.5)