FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005331463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123751252
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG242750.023.47803743
CGTATGC258200.022.07315444
CTCGTAT260650.021.72695442
TCTCGTA294850.019.35933341
AATCTCG295250.019.24930439
GATCGGA295750.019.063951
ATCTCGT321750.017.81774940
ATCGGAA327350.017.2499522
ACACGTC349200.016.86541413
ACGTCTG371500.015.92575715
GTATGCC362350.015.87100445
CGTATCG54350.014.7512763
CACGTCT402850.014.65284714
TCGGAAG398300.014.4859823
CACACGT422750.014.02695312
GCACACG456000.013.30015311
CGTCTGA449300.013.107738516
CGGAAGA491550.012.2551724
TTGAATC489000.012.03645336
CCCGTAT96350.011.9700241
Produced by FastQC (version 0.11.5)