FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56827705
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG35700.012.72888912
CGACCGT19500.012.5937281
TACCGTA41100.012.1510219
CCCGTAT52450.011.7697581
GTATCGA44300.011.2367524
CGTATCG40950.011.1664963
CCGTATG41450.010.90883811
ACCGTAT41850.010.75073310
TATACGG36750.010.528789513
GTATACG37500.010.43819312
CGGGTAT23100.09.4606761
GACGCTA42450.09.1795344
CGCGTAA5659.094947E-129.1715311
TAAGGCG79300.09.1344498
CCGTATC58550.09.0796422
CTACCGT58150.09.0137598
CCGGTAA28050.08.9156411
TCGTATG32300.08.777184545
CTGGAAT271900.08.7004661
CTTGGAT219200.08.66461851
Produced by FastQC (version 0.11.5)