FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39427371
Sequences flagged as poor quality0
Sequence length30-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA1277690.32406167786333No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1113210.28234446572661415No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCG1109140.28131218792143153No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGAC835320.21186297204548585No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACG773200.19610741989365713No Hit
GGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGG640920.16255712307067088No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC625350.15860808979629912No Hit
GTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGA505330.1281673079343789No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGC438950.11133128810439834No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGG434870.1102964739901121No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGC433700.10999972582498589No Hit
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCT422680.1072047131927716No Hit
GGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGG415310.10533545338338689No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGA412550.10463543207078149No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC407010.1032303168273634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA150450.029.789027
ACGTTCG153050.029.4874926
CAGACGT156100.028.868233
AGACGTT158700.028.1978934
GCAGACG161000.028.0865572
GACGTTC160500.028.0629945
GGGTCGT168950.026.26241916
TCGAATG174650.025.89207310
TTCGAAT178850.025.572119
GTTCGAA178550.025.4020378
TGGGTCG177400.025.09925715
CTACCGG103750.025.03163742
GTCGTCG176400.025.0264218
GGCAGAC199900.024.9317051
GCTACCG104850.024.21275341
GTCGGAT46350.024.0074621
TACCGGC109800.023.62761943
CGAATGG196100.023.10556211
ATGGGTC196500.023.03555114
TCGGATT52000.021.686292
Produced by FastQC (version 0.11.5)