FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37434609
Sequences flagged as poor quality0
Sequence length30-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAC36350.021.90020245
TACGGTA42200.018.98837744
CTTACGG44000.018.25323542
CCTTACG44700.017.884841
TTACGGT46000.017.57345643
CGAGACG23250.017.17093845
GACCGCG45800.016.81403532
AAGCGCG13400.016.39728439
TGCGGAG69900.015.84487944
TGACCGC50450.015.61042831
TACGCCG39900.015.59418221
TAGCTCG34050.015.51226224
ATGCGGA72600.015.28661643
TTATGCG73200.015.07905741
GTGACCG51950.015.03801130
GCGTTGG32850.014.96336639
CGTCGTG17700.014.92077314
TATGCGG73550.014.78642142
CCGCGGT54000.014.57165434
ACCGCGG53150.014.54032733
Produced by FastQC (version 0.11.5)