FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47358232
Sequences flagged as poor quality0
Sequence length30-51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCG116050.020.2156321
TATTGCG47600.018.79309842
TAGCTCG67950.018.15194524
TTACGCC133000.017.84117720
TTTACGC135050.017.58693319
ACTTGCG80500.016.2958667
GCGCTTA80850.016.14159211
GCGGTAC57650.015.91793429
CTTTACG151750.015.75477918
GATCTAA172350.015.6772325
CTTGCGC84700.015.56708058
AGCTCGT80800.015.46506425
CGCTTAC84400.015.46268412
ACGGCCG40600.015.14575145
GGGTGAT212650.015.0083351
TGCGCTT90100.014.80801210
CGGATCG13050.014.5802313
TGATCTA182650.014.4990154
GTCGGAT14050.014.3466071
GTCGGCT29400.014.3216671
Produced by FastQC (version 0.11.5)