FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46362430
Sequences flagged as poor quality0
Sequence length30-51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG26250.024.364986
TACGCCG96300.021.99060221
TATTGCG54200.021.69106342
TAGCTCG76900.021.1696824
GCGGTAC57200.020.23682829
TTACGCC108600.019.97355520
TTTACGC108850.019.88654919
GTCGTCG32150.019.75380718
GGGTCGT34950.018.49079916
AGCTCGT90150.018.1976125
TTATGCG105900.018.04341738
TATGCGG105700.017.77473839
ATGCGGA108250.017.2502540
CTTGCGG68450.017.01816226
TGGGTCG38700.016.87240215
GCACGAA163100.016.84106845
ACGCCGG123750.016.805622
GATCTAA145100.016.7682275
TGACGCA114350.016.72776228
CCTTGCG71000.016.70681425
Produced by FastQC (version 0.11.5)