FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30340508
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGA662110.218226405437905No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA545160.1796805775302114No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT367130.12100324753955999No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAG365220.12037372610900253No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGG355280.11709757793112757No Hit
GCCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGA338700.11163293640304242No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT337650.11128686441242183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32500.036.20653545
CGGAGGA141750.023.1756643
ACGTTCG4750.022.254126
GCGGTAC42200.022.2255629
TAGCTCG51650.022.16548524
TATTGCG44750.021.11904742
AGCTCGT57300.020.09764325
ACGGTAC48150.019.81245245
GCTCGTC58500.019.57000526
TCTCGTA63150.019.12404841
CGTATGC64050.019.00583344
TCGTATG64950.018.8114843
TACGGTA50800.018.78066344
TGCGGAG173550.018.72100341
ACTTGCG29600.018.6157517
CCTTACG52150.018.4485441
TGCGGTA51350.018.44035528
ATGCGGA177400.018.40395440
CTTACGG52200.018.32046542
CTTGCGG51650.018.24621426
Produced by FastQC (version 0.11.5)