FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31121328
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC655330.2105726336613913No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGA428050.13754233109846725No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAG393940.12658200189914773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGA160300.020.57630216
CGGACAC161850.020.4070513
GCCCGGA164100.020.18215210
GGACACG165050.020.05229214
CGCGTAA103300.019.8770731
ACGGACA167750.019.67636718
GCGTAAC112450.019.3079172
CGGACAG175450.018.81288719
CTCACCC177050.018.7440592
TGTGTCG44400.018.5466583
TGTCGAG45400.018.0886425
TAGTTAG123450.018.0066999
CGGTAAA111000.017.91827213
TTATCCG111950.017.7260828
GATTGAC186300.017.64536726
CCGGACA189800.017.63898512
GGATTGA189150.017.4032925
TCCGGTA114850.017.35679611
CCTCACC195600.017.32311
TTCGCGT3900.017.318671
Produced by FastQC (version 0.11.5)