FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35786341
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1367200.38204520545981496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG24400.027.6628593
GCGTACG24800.027.035242
TATGCCG16400.026.06828345
GGCGTAC29100.024.7537231
TCGTATG40500.021.11235443
GTACGGA33700.020.1619994
CGTTCGA7750.019.4503147
TCTCGTA45100.019.25842341
ACGTTCG8100.018.8876026
GTCACGA46000.018.78169629
TCGCGCG21350.018.75965145
CGTATGC46300.018.71047644
ATCTCGT47350.018.48606340
ACCGCGT14800.017.7859486
CGTCTGA48700.017.74050916
TCACGAT49400.017.30803730
AGTCACG50600.017.20773328
CCGCTCG38950.017.1558528
GGTCGAC39550.017.0749051
TACGGAA40450.016.9086365
Produced by FastQC (version 0.11.5)