FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65048008
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC1407220.21633560246764205No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACG1023640.15736684819003222No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC993440.15272412338898986No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAG952890.14649026608163004No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC901780.13863299241999846No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCC882350.13564596782118218No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCG874840.1344914359252938No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA863320.13272043626608826No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC804630.12369786942591694No Hit
GTATAATACTAAGTTGAGATAATATCATTTACGGGGGAAGGCGCTTTGTGA769080.11823267516508731No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGA738940.11359917432060332No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGC731350.11243234381597052No Hit
GGGTATAATACTAAGTTGAGATAATATCATTTACGGGGGAAGGCGCTTTGT713100.10962672369613533No Hit
GGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGG668360.10274872675578321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG195800.029.3819626
TCGTATG95600.028.55032243
CAGACGT214050.027.12943
AGACGTT214000.026.988344
GCAGACG220450.026.5663222
GGGTCGT221850.025.69840816
CGTTCGA224850.025.5755717
TGGGTCG223150.025.52853415
TATCTCG108300.025.03569839
GACGTTC233250.024.87675
GTCGTCG229650.024.86475818
TCGAATG242400.023.99337210
GTATGCC116000.023.66484345
TCTCGTA116300.023.37189941
GTTCGAA253200.022.9610738
CGTATGC119150.022.75576644
GGCAGAC282550.022.7149471
TTCGAAT265750.022.1984589
ATGGGTC265600.021.78751214
GCGATCG262450.021.34811641
Produced by FastQC (version 0.11.5)