FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41335834
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2220150.5371005699316481No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG971620.2350551340031025No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGC899170.21752796859015838No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACC770700.1864483972913187No Hit
GCCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGA756760.18307602067494272No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA674680.16321915749903584No Hit
CCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAA634730.15355441963503141No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGA595020.14394774277446537No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC576740.13952542967924636No Hit
GTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTGTGTCAACTGGAGT567660.1373287883824964No Hit
GTGAGGTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGAT562290.1360296734305639No Hit
GGCTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGATGCTTGTCCC548800.13276616119563475No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAG547180.13237424942242607No Hit
GGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATT541870.13108964972135315No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT535220.12948087608441625No Hit
AGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCA511560.12375702882878811No Hit
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCA504290.12199826426630221No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTA470700.11387214299341342No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA468350.11330362900141316No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACT455860.11028203761414371No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGG441080.10670644748573357No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG439370.1062927628362355TruSeq Adapter, Index 7 (97% over 36bp)
GTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCT422870.10230106884985071No Hit
GGCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAG417370.10097050418772244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG61900.032.67976841
AGTCACG67500.030.33205228
GTCACGA67450.030.28859529
CACGAGT67800.030.23168431
CGAGTGG67850.030.20966133
TCTCGTA68400.029.8704543
ACGAGTG71500.028.6360232
CGTCTGA74050.027.5881716
ATCTCGT74250.027.27454642
TCGTATG74900.027.09741245
ACACGTC75650.027.03517213
ACGTCTG80050.025.68898815
CTCGTAT85800.023.70687144
CACGTCT90550.022.76052314
CACACGT92800.022.2571712
TCACGAG96250.021.52950930
GCTATCA207700.021.27732543
AGCTATC210250.021.10485642
GGATATC96900.021.06172438
GCACACG99450.020.99512311
Produced by FastQC (version 0.11.5)