FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36549101
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1432580.39196039322554066TruSeq Adapter, Index 8 (100% over 51bp)
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCG440630.120558368863847No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA426100.11658289488433654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28450.036.93236545
TCGTATG181000.036.361443
TCTCGTA184900.035.40032641
ATCTCGT188000.034.7927140
CGTATGC190050.034.5111444
GTATGCC195450.033.45279745
CGTCTGA202250.032.49501416
ACGTCTG219650.029.97154215
CTCGTAT219400.029.87438442
AATCTCG221350.029.73367539
ACACGTC221500.029.71028913
CACGTCT230600.028.55810514
GAATCTC239250.027.63134838
CACACGT242750.027.29593712
TTGAATC246100.026.91637436
CAGTCAC252200.026.47823327
GTCACAC252050.026.39651929
ACACTTG254750.026.16949532
CGTTCGA41700.026.0604467
TGAATCT257150.025.67221537
Produced by FastQC (version 0.11.5)