FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41225041
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG44600.026.93991345
TATCTCG44950.026.43198841
TCCGTAT47400.025.15916837
TCTCGTA48400.024.77911843
ACGTTCG24900.024.3070766
CGTTCGA25950.023.3230727
CCGTATC51850.022.6970638
AGTTCCG53400.022.66869734
ATCTCGT54150.022.23090742
CGTATCT55550.021.59035539
TTCCGTA59250.020.35499236
CGTCTGA59500.020.343316
CCCGTAT19200.019.5763151
ACACGTC64600.019.12032913
GTCGTCG31250.018.71924618
CGTATCG8450.018.640273
CCCGTCT115900.018.5842151
ACGTCTG68450.018.0120315
GACGTTC36100.017.7639485
ATGGACC124850.016.58063542
Produced by FastQC (version 0.11.5)