FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29551503
Sequences flagged as poor quality0
Sequence length51
%GC65

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGC16271455.506132801434838TruSeq Adapter, Index 18 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG13654684.620638077190185TruSeq Adapter, Index 18 (97% over 40bp)
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCG2966321.0037797400693969No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2767120.9363720011127692No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTC1652000.5590240198611895No Hit
GGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCC1249990.4229869458754771No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGT1157110.3915570724101579No Hit
GGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGG1057390.3578125958601835No Hit
GGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGG995430.3368458111927505No Hit
GGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGA914360.30941235036336395No Hit
GTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGA897140.3035852355800651No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGC819810.2774173618174345No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC723270.2447489726664664No Hit
GGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCG707620.23945313373739402No Hit
GGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCG700400.23700994159248007No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC684170.23151783515038138No Hit
GGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACA658300.22276362728487956No Hit
GGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACC613540.20761718955546862No Hit
GGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCC609910.2063888256377349No Hit
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGG593740.20091702273146647No Hit
GGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCC590490.19981724787399138No Hit
GGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGA571530.19340133055161357No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGG567960.19219327016971016No Hit
GGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGA546770.1850227380989725No Hit
GGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGAC543640.18396357031315802No Hit
GTGGGGGTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTT534450.18085374540848229No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA520560.17615347686376562No Hit
GGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCC513450.17374750786787393No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA497610.1683873744086722No Hit
GCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCC495560.1676936702678033No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCGC493310.16693228767416668No Hit
GTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCG425170.1438742388162118No Hit
GGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCC420080.1421518221932739No Hit
CGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGA416950.1410926544074594No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA399540.13520124509403125No Hit
GCCCGCCGCAGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAG388850.13158383179359778No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGT386570.130812297432046No Hit
GGGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAG372740.12613233242315966No Hit
CCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGC360850.12210884840611999No Hit
CCCGACCCTTCTCCCCCCGCCGCCGCCCCCACGCGGCGCTCCCCCGGGGAG359470.12164186708202288No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTCC350660.11866063123760576No Hit
GCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCGT346240.11716493743143962No Hit
GGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAG333830.11296548943720393No Hit
CGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCC333210.11275568623362406No Hit
CCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCC331900.11231239236799563No Hit
GTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCCCCCACCGT325870.11027188701704953No Hit
GGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAGGAAAG318040.10762227559119412No Hit
GGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGC317650.10749030260829712No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC315400.10672892001466051No Hit
GGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTC315130.10663755410342411No Hit
GCCGCAGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGC315070.1066172505675938No Hit
GTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACC312460.10573404675897533No Hit
GGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCC310300.1050031194690842No Hit
GTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGAC307520.1040623889756132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1973150.044.27131745
CGTATGG15600.042.40491545
GTTACGT31800.041.60257328
TTACGTC30300.040.98891429
TACGTCC29950.040.641730
CGTATTC26000.039.63561645
CCGTATG8250.038.1830444
GTCGTAT4900.037.65426643
GTTCGCA13850.037.36347633
CGTCTTA16550.037.24999215
ATATCGT8850.037.1197741
ACGTTTG20500.037.096814
ATTTCGT34400.036.36738241
CGTATCC4800.036.09465845
CATTTCG34950.036.05259740
ATCGGAC9850.035.881151
CAAACGT11350.035.87989811
TTCGCAC14900.035.787534
CGGACAT11600.035.300736
GTATGCC33300.035.2711645
Produced by FastQC (version 0.11.5)