FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52885934
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCG1652150.31239875616075913No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGC1565650.2960428003408241No Hit
GGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGG1298120.24545657073958455No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACG1072940.20287814147330746No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG1008670.19072557175599847TruSeq Adapter, Index 21 (97% over 40bp)
GGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGA963500.18218454835268674No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGC912260.1724957717490628No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGA867400.1640133650660306No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGG818090.15468952481769538No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA815620.1542224819174036No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC751850.14216445529731972No Hit
GGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGG747950.14142701913896424No Hit
GTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGA690240.13051485485724806No Hit
GTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGA609690.1152839618942912No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG549900.10397849832811878No Hit
GGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGA541430.10237693826112629No Hit
GGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACA536070.10136343625887369No Hit
GGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCC531720.10054091131301568No Hit
GTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGGCGGCCGCGACG529270.10007765013661288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG140900.036.161445
CGTTTCG144600.034.8880233
TCGGAAT150500.033.57936537
TCTCGTA149650.033.41855643
TTTCGGA154450.032.69138735
AGTCACG160050.031.83789628
ACGTTTC159900.031.7186832
GTCACGT160900.031.69753529
GTCGGAT90800.031.2146971
ATCTCGT161500.031.00771742
TCACGTT170800.029.99192430
CACGTTT171950.029.8077131
TCGGATT94550.029.7296812
TTCGGAA172400.029.36609836
AATCTCG174850.028.67670441
ACGTTCG162700.028.112236
CTCGTAT183350.027.39890344
CGTTCGA170300.026.844467
CAGACGT173200.026.6293373
AGACGTT177250.025.8052064
Produced by FastQC (version 0.11.5)