FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8981670
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG136540.15202072665773736TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG20600.034.0786945
CTCGTAT28850.024.48796844
TCTCGTA31650.022.6055543
ACGTCCG33850.021.60138132
ATCTCGT32900.021.5415242
CGCACAT34050.021.07804937
AGTCACG34350.021.0268328
GATCGGA35000.020.9027921
GTCACGT35700.020.35593829
ATCGGAA37400.019.6128862
CGTCCGC37900.019.47114633
GTCCGCA37450.019.46480634
CACGTCC40650.018.4859931
ACGTCTG39200.018.42365615
ACACGTC39350.018.41111413
CATCTCG39400.018.27323541
TCCGCAC40750.018.05414635
TCACGTC41050.017.81258830
CCGCACA41550.017.43579136
TCGGAAG43750.017.0233673
Produced by FastQC (version 0.11.5)