FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005331523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63302140
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGA4185120.6611340469690282No Hit
GGGTGATCTAAAACACTCTTTACGCCGGTTTCTATTGACTTGGGTTAATCG2667630.42141229348644454No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACC2221830.35098813405044443No Hit
CTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTATGCAG1990690.31447436058243844No Hit
CTAAAACACTCTTTACGCCGGTTTCTATTGACTTGGGTTAATCGTGTGACC1768580.2793870791729948No Hit
GGCTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGATGCTTGTCCC1590090.25119056006637375No Hit
AAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTT1571200.24820645873899366No Hit
ATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCC1567750.24766145346744994No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCC1517710.2397565074419285No Hit
CCGGTTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAA1516830.2396174916045492No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA1478660.23358767965822327No Hit
GGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT1400040.22116787836872495No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT1313500.20749693454281326No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAG1301790.20564707607041408No Hit
GGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTA1227740.19394920930003315No Hit
CTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGATGCTTGTCCCTT1173260.1853428651859163No Hit
CGATTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTT1045430.16514923508115206No Hit
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCA1014570.16027420242032892No Hit
GCCGGTTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGA1008420.15930267128409875No Hit
ATCGATTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGGTCC999780.15793778851710227No Hit
ATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATC974170.15389211170428047No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAG910190.14378502843663737No Hit
ACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC902810.14261919107316118No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGG896750.14166187746575393No Hit
GTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTTT870570.13752615630372053No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAAC866730.136919541740611No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA813570.12852172138256304No Hit
CGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT792480.12519008046173477No Hit
TATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA771740.12191372993077328No Hit
TGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGAATGTTT762410.12043984610946802No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCAT728850.11513828758395846No Hit
GGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCA727210.1148792126142971No Hit
GTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTAT718290.11347009753540718No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC672400.10622073756116303No Hit
TTTATCGATTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCAGG663580.10482741973652077No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAG652920.10314343243372183No Hit
CAGATATGTTAAAGCCACTTTCGTAGTCTATTTTGTGTCAACTGGAGTTTT637180.10065694461514256No Hit
GCTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGATGCTTGTCCCT635170.10033941980476489No Hit
GTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCT633100.10001241664183864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)