FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30798339
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG398830.1294972433415971No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA317830.10319712371501594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG12050.027.0792832
CGTACGG12850.025.5670033
GGCGTAC16150.021.8777331
ACCGCGT12700.018.6018926
GTCGATT14150.018.1310791
CCGCGTT12950.017.895337
CGTATCG4750.017.0544993
GGAACGT45150.016.84326625
TTTACGC141450.016.68568819
TTACGCC141400.016.67570320
GGGTGAT180850.016.6748351
TACGCCG141150.016.65736421
CCCGTAT12450.016.6300561
TTCGTAG87600.016.1038923
CGTAGTC87950.015.886312525
CCCGTCT86950.015.8141861
GAACGTG48850.015.56752126
GATCTAA166750.015.4497615
CGCCAAT14750.015.40664133
GTACGGA21900.015.3082564
Produced by FastQC (version 0.11.5)