FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30121985
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC565320.18767687454860627No Hit
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTT477230.1584324539036853No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGA398280.1322223618396995No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGAC369310.12260480177518182No Hit
GGGTGATCTAAAACACTCTTTACGCCGGTTTCTATTGACTTGGGTTAATCG367910.12214002496847402No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCC356950.11850148653881874No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA348910.11583233973458257No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC344930.114511045669799No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACT323980.10755599274085025No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAAC323810.10749955555717859No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA316020.1049134046112831No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGA307690.10214798261137172No Hit
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCA301830.10020255969186627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG87950.026.576246
CGTTCGA92850.025.07767
TATTGCG59050.024.51196942
CAGACGT96500.024.4317763
GCAGACG97450.024.0562442
GACGTTC100450.023.4706135
ACCGCGT13350.023.4232626
AGACGTT101100.023.1637614
GTCGTCG100550.022.88877518
GGGTCGT102800.022.49767516
TGGGTCG103250.022.4214515
TCGAATG107250.021.96265410
GTTCGAA108200.021.7694248
TTCGAAT111250.021.4354889
CGAATGG110150.021.24145711
CCGCGTT14950.020.7666027
CCTTGCG75650.020.01425725
TACCGGC54550.019.89021343
CTACCGG55300.019.86469342
GCGGTAC73850.019.86454829
Produced by FastQC (version 0.11.5)