FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39934695
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG40450.027.6410436
CGCCAAT36450.027.3454333
TATCTCG37500.027.07263639
TCTCGTA38050.026.26718941
GTCACGC38250.026.23505629
TCGTATG38400.026.08639543
TATTGCG84450.024.54964642
CGTTCGA45550.024.5471087
GTCGTCG45700.023.72805218
ATCTCGT43750.023.10221140
AGTCACG45000.022.39979628
ACGCCAA46100.021.91411832
GCGGTAC94050.021.7940629
CGTATGC47800.021.05062144
TGGGTCG53300.020.97808615
GACGTTC55050.020.7188995
CCTTGCG102250.020.4622625
GGGTCGT55000.020.20695716
CTTGCGG105300.019.80547726
GCGATCG53500.019.77558541
Produced by FastQC (version 0.11.5)