FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24279169
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC324170.13351774931011848No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGAC275540.11348823347290017No Hit
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTT266600.10980606461448496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG36350.023.5178186
ACCGCGT15750.022.1395636
CGTTCGA38750.022.120067
CCGCGTT16450.021.3349597
TATTGCG22950.018.93009842
TGGGTCG45300.018.92180415
GTCGTCG45300.018.9215318
GGGTCGT45000.018.89796416
GACGTTC46450.018.6947025
GTCGGAT18150.018.6038061
GAACGAT20850.018.56951745
AGACGTT48150.018.0345524
GTTCGAA48150.017.9886078
TTCGAAT50500.017.5079089
CAGACGT50900.017.4143053
TCGAATG49550.017.3897610
AGACCGC20600.017.3637774
CGAATGG50700.017.30593711
CGCGTTC20200.017.2628868
TACCGGC35900.017.24314543
Produced by FastQC (version 0.11.5)