FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52574031
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG15450.017.784043
CCGCGAC45850.016.48799945
TATTGCG76950.016.22751242
TACGCCG161750.016.16017221
GATCTAA200800.015.852015
ACGTTCG18000.015.7467046
TCGGATC24800.014.8029632
TGACGCA166250.014.72635228
TGATCTA210900.014.6826944
CTGACGC166450.014.640964527
TCTGACG167550.014.59856526
CGACACG63400.014.51454141
TTACGCC188700.014.29326420
TTTACGC189150.014.2354619
CCCGTCT118450.014.1659181
CCCGTAT17200.014.142261
TTATGCG174050.014.07747438
TACGTTC110100.013.93869427
ACGGCCG67650.013.93523345
CCCTACG109300.013.93325824
Produced by FastQC (version 0.11.5)