FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36579769
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA478720.13087015393672935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCT39200.023.01684641
CGGAGCG43550.021.59649839
GCGCTGA42400.021.54461743
AGCGCTG46100.019.96210342
CGCTGAG47400.019.41419644
GGAACGT83950.019.26985525
GAACGTG86950.018.65651926
ACGTGAG89350.018.0294928
AACGTGA90950.017.7370527
TTTACGC101050.017.14461319
TTACGCC101650.017.08768320
ATACCGC32400.016.80487627
GGGAACG96950.016.7323824
CCCGTCT91600.016.6594731
CCCGTAT16350.016.6569181
CTGGCGG56900.016.25242835
AATCGCA88900.016.19738845
CGTCTCT73750.016.1394543
CGAGCCG33800.016.04227315
CCTTGCG60500.015.91690825
Produced by FastQC (version 0.11.5)