FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30483912
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG321690.10552779446417508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG46850.024.06054942
TTGCGCC52200.021.55104444
TGCGCCA57950.019.45143745
TACGCCG88850.019.4225121
CCCGTAT12850.019.2654021
CTTGCGG61350.018.92345426
CCTTGCG61250.018.80741725
TGCGGTA61250.018.80738428
GCGGTAC62100.018.33257529
TTGCGGT63350.018.29048327
CCCGTCT67300.018.0244581
AATCGCA87900.017.71269645
ACGCCGG97600.017.45072422
CGGTACT65950.017.43294130
TTACGCC101900.017.17800920
TTTACGC103250.017.0405419
GTCCGTT8150.016.8446031
GTCCGAT5750.016.4387951
ATTGCGC71950.016.19829243
CCCCACT143250.015.7106411
Produced by FastQC (version 0.11.5)