FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31257636
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC918520.293854596041748No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG830730.2657686588966613No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA714690.228644930154027No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGA697790.22323825128682157No Hit
GTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCT656540.21004147594526984No Hit
GGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT601150.19232100597754737No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT528220.1689891071736839No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA527390.16872357205772057No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACC483100.15455423436372476No Hit
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCA446940.14298586111886388No Hit
GCCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGA435860.13944112728166647No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAG400790.12822146882764904No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC387080.12383534058685693No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGG367500.11757127122473371No Hit
CCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAA358530.1147015724413708No Hit
GGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTA346860.1109680847265609No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGG332660.10642519479080248No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCA329020.1052606793424813No Hit
GGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAG324830.10392020688960611No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC324640.10385942174257837No Hit
GTTCAGATATGTTAAAGCCACTTTCGTAGTTTATTTTGTGTCAACTGGAGT315220.10084575813730763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG22650.028.5979296
GTCGTCG23600.027.54380618
TATTGCG78850.027.30272742
TGCGCCA85550.025.29622345
TCGTCGC26700.024.59832419
TTGCGCC88850.024.3064844
CGTCGCC27350.024.01372120
TCGCCGC27300.023.9760822
CGCCCCT44350.023.93816
CCTTGCG91650.023.86722625
CTTGCGG90850.023.85712826
CGTTCGA27250.023.8520097
GACAACC110800.023.34695635
GTCGCCG28150.023.17202421
TACGCCG189650.023.15108521
TTGCGGT94050.022.97364627
GCTATCA113250.022.92308843
CGATTAG49850.022.9219914
ACGCCGG189250.022.87929722
GCGATCG27150.022.78550541
Produced by FastQC (version 0.11.5)