FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34904330
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCA442030.12664044833406057No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG420810.12056097338066653No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCA387400.11098909504923887No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC360210.10319923058256669No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG359490.10299295245031204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG30650.026.3494136
TATTGCG90850.024.77169242
CGTTCGA35100.022.9447277
GTCGTCG37800.021.12776418
TTGCGCC108000.020.92079244
CCTTGCG112900.020.16778825
TGCGCCA112050.020.12439245
GACGTTC42850.020.0024995
CTTGCGG115650.019.63140726
GGGTCGT41050.019.56436516
GCGGTAC115750.019.47962829
TACGCCG117200.019.44570221
TTGCGGT116650.019.30929827
GGTACTA122550.019.0596231
TGGGTCG43050.018.70757515
TGCGGTA123250.018.36725828
TACGTTC117550.018.14691527
ATGGACC115750.018.06239542
CCCTACG118250.018.01837224
ACCGCGT8750.017.996876
Produced by FastQC (version 0.11.5)