FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32632813
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG22400.020.0843936
TACGCCG99600.018.04513521
TATTGCG33250.016.57762742
CGTTCGA27450.016.3894237
GATCTAA133350.016.1939545
TTTACGC116750.015.74120219
TTACGCC117900.015.58766220
CCCTACG91300.015.42699824
GGGTGAT141550.015.4184241
CCGGTAT90450.015.3639411
ACGCCGG117950.015.31410622
CGACACG47500.015.29894541
TACGTTC93250.014.98655627
TGATCTA143350.014.9410424
GTCGTCG29300.014.81707318
CATCCGG25100.014.78981841
CCTACGT94750.014.7719225
ATCCGGA24700.014.7559142
CTACGTT95600.014.66524226
GGTGATC132850.014.5968452
Produced by FastQC (version 0.11.5)