FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49007302
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1132100.23100639165975714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG15850.026.1693972
CGTACGG16550.024.7900623
ACCGCGT21900.020.9533716
GGCGTAC22600.020.4483621
CCGCGTT23250.019.7367257
GTCGCGA2251.8189894E-1217.0325431
GGAACGT65950.016.09940125
GTCGCAT10550.015.3848471
GCCGTAT14300.015.2914551
CGCGTTC30600.015.290088
CCCGTAT17550.015.1571661
GACCGTC71650.014.66567144
AGACCGC32300.014.6559934
TAGACCG72150.014.59367542
GAACGTG74200.014.55193226
CGGAGCG39150.014.42454139
CCCCACT189550.014.3547811
CCCGTCT125600.014.3048011
TACGTTC89300.014.28285427
GTACGGA29800.014.2213264
Produced by FastQC (version 0.11.5)