FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20304322
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGC314840.15506058266806447No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG309020.1521941978658534No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG266300.13115434241044838No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG250550.12339737322920707No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGC230230.11338965172045636No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTA219650.10817893845458124No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTT215170.10597251166525039No Hit
GGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGA211900.10436201711143075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCT38600.030.76435941
CGGAGCG42000.029.18395639
AGCGCTG42150.028.49344342
CGCTGAG44600.028.08639144
GCGCTGA47300.026.2945243
GGAGCGC47800.025.12549440
CTGGCGG51900.024.65557535
GCGGAGC50350.024.4780638
CGCGTTT3150.020.9715731
CCTGGCG62850.020.71779434
CGCGTAT1552.0008883E-1120.5751
TGGCGGA58800.019.81174336
ACGGCCC65550.019.45273229
GTCGGCC67750.018.55564121
CGGCCCT68950.018.2651830
TACGTTC45300.018.0210827
GCCCACG75100.017.69782625
CTACGTT47550.017.49941826
CCTACGT47400.017.31756825
CCCACGG76650.017.28126326
Produced by FastQC (version 0.11.5)