FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29320602
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGC372010.12687665826233718No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCG328620.11207818993620937No Hit
GGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGA297470.10145426072766173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCT34350.026.18940441
CGGAGCG35750.025.10076739
AGCGCTG38350.023.75100742
GCGCTGA40600.022.93330843
CGCTGAG41100.022.92693744
CTGGCGG48900.019.22375935
GGAGCGC47500.019.0337740
GCGGAGC52000.017.81899638
CGCGTAT2105.456968E-1217.3585281
GTCGTCG17500.016.96330618
CCTGGCG59100.016.09621434
TTCGTGA37650.015.70958144
CCGGTAT71450.015.6882711
TGTGGCG172600.015.15339129
TACGTTC51600.015.07993727
GTCGGCC61900.015.07756821
ACGTTCG20300.014.9573466
ATACCGC26200.014.84970927
CCTACGT52400.014.80678925
ATTCGTG39800.014.69205843
Produced by FastQC (version 0.11.5)