FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22705362
Sequences flagged as poor quality0
Sequence length51
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCG12322035.426925146579913No Hit
GCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCC7368253.2451585665095317No Hit
CTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCC7099253.1266843488335487No Hit
GGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCG5367212.3638513228725446No Hit
GGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGG4827962.1263523567692957No Hit
CCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGA4789162.109263882249488No Hit
GTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGT4065051.7903480244005798No Hit
CGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCC3585661.5792128749147447No Hit
GGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGC3504861.5436265671518472No Hit
CGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCG3382591.489775851184403No Hit
GTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTC3281541.4452709452507297No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2655691.1696312086986325No Hit
CCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCC2418871.0653298546836645No Hit
CGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCG2391721.0533723267658097No Hit
CTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGGG2159160.9509471815512125No Hit
CCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGC2146280.9452745126899981No Hit
CGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGG2122900.9349773855180111No Hit
GGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCC2116600.9322027105315476No Hit
CACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGAC2060840.9076446347783399No Hit
CGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCT2003180.8822497522831831No Hit
GCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCC2001560.8815362644295212No Hit
GGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCC1988080.8755993408076911No Hit
GTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTC1976490.8704948196818003No Hit
CCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGAC1801640.7934865781924112No Hit
CTCCGCCGCCCGCCGCCGCCGCCGCGCGCCGAGGAGGAGGGGGGAACGGGG1705250.7510340508995188No Hit
CCCGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGCGGCGGCG1535520.6762807833673825No Hit
GCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGG1449180.6382545233148011No Hit
CGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCT1423050.6267462284899928No Hit
CGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGC1382950.6090852019888517No Hit
CCACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGA1330250.5858748255147838No Hit
CCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACG1270510.5595638598494929No Hit
CCCGGCCCCGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGCG1223250.5387493932050059No Hit
CCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGC1211710.5336668933091664No Hit
GGCGTCGCGGCCGCCCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCCCCC1156150.509196902476164No Hit
CCGCCACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGA1077290.47446501843925676No Hit
CGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGG1069150.4708799621869054No Hit
GACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGG1029870.4535800838586057No Hit
CCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGG1004830.44255185184891566No Hit
CCCGCCGCCGCCGCCGCGCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACG988230.4352408034718847No Hit
GCCCCGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGCGGCGG903890.3980953926213553No Hit
CCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGG874210.38502359046290474No Hit
CCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGG844900.37211474540683387No Hit
CGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGG820490.36136398089579014No Hit
CCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGG819040.36072536522430254No Hit
GTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGA810410.3569245009174485No Hit
CCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGG797520.35124742781022383No Hit
GCCACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGG748000.3294376015674183No Hit
CGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGA747350.3291513255767514No Hit
ACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACG719080.31670052210574756No Hit
CCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGG713920.3144279311644536No Hit
CGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCC684650.30153670309242375No Hit
GTCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCC674040.2968637980755383No Hit
CGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCG658140.28986104691922554No Hit
CGCCACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAG630720.2777846043590937No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT621610.2737723362437472No Hit
CCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCC606290.2670250313560295No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG593510.2613964049549177TruSeq Adapter, Index 27 (97% over 44bp)
CCGCCGCCCGCCGCCGCCGCCGCGCGCCGAGGAGGAGGGGGGAACGGGGGG582190.2564107984713038No Hit
CGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGA548080.24138791533030832No Hit
CCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCC534030.23519994968589358No Hit
CGGCGGTGAACGGGGAGGAGGCGGGAACCGAAGAAGCGGGGCGCGCCGACC532410.2344864618322315No Hit
CGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAG530630.23370250604240533No Hit
CTCCCCACCCCGCGCCCTCTCTCTCTCTCTCTCCCCCGCTCCCCGTCCTCC526680.2319628288683528No Hit
GGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG519540.22881819721702742No Hit
GTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGCGGCGGCGGGC512290.2256251188595892No Hit
GGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCG504800.22232633859790474No Hit
GCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCG498290.21945917444522578No Hit
CCCCGGCCCCGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGC497710.2192037281766307No Hit
GGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGC483060.21275150777160037No Hit
CCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGG479440.2111571707158864No Hit
GTCTCGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTCGGGCGGCGGCG455660.20068387370348906No Hit
GCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGC454130.20001002406391932No Hit
GGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCC442730.1949891836122234No Hit
GCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGG441560.19447388682902303No Hit
GTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCC437670.19276063513103203No Hit
GGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCTCCCC431150.1898890667323428No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTC430800.18973491812198368No Hit
CTCGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTCGGGCGGCGGCGGT415820.18313735759861482No Hit
CGCCGCCCGCCGACCGCCGCCGCCCGACCGCTCCCCGCCCCCAGCGGACGC410860.18095285157752605No Hit
CGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGG409620.18040672507225386No Hit
CTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGGGCGC401120.17666311596353318No Hit
GCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAG381210.16789426215710632No Hit
CCCGCGCGCGCCGCTCGCTCCCTCCCCACCCCGCGCCCTCTCTCTCTCTCT377940.16645407371175144No Hit
CCGGCCCCGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGCGG376820.16596079815860237No Hit
CCCCACCCCACGTCTCGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTC375440.16535301220918652No Hit
GGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCC360530.15878628140788945No Hit
CCCCGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGCGGCGGC359060.15813885724438131No Hit
CGCCGACCGCCGCCGCCCGACCGCTCCCCGCCCCCAGCGGACGCGCGCGCG358710.15798470863402223No Hit
CCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCC354180.1559895851913746No Hit
TCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTC343000.15106563815190438No Hit
GTCCCGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGCGGCGG341960.15060759656683737No Hit
CGCCGCCACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGG339250.149414045898057No Hit
GTCGCGGCCGCCCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCCCCCCCC337220.14851998395797433No Hit
CGCCGCCGCCGCCGCGCGCCGAGGAGGAGGGGGGAACGGGGGGCGGACGGG335980.14797385745270214No Hit
CCGCCCGCCGCCGCCGCCGCGCGCCGAGGAGGAGGGGGGAACGGGGGGCGG330990.1457761386935826No Hit
CCGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGCGGCGGCGG330150.1454061820287208No Hit
GGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGC325240.14324369723768332No Hit
CGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCTCCTCCT324460.1429001660488831No Hit
GTCGCGGCCGCCCCCGGGGAGCCCGGCGGGCGCCGGCGCGCCCCCCCCCCA315320.1388746851955058No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG310500.1367518386185607No Hit
GCCGCCGCCCCGACCCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGG302660.13329890974651715No Hit
CCTCCCCACCCCGCGCCCTCTCTCTCTCTCTCTCCCCCGCTCCCCGTCCTC301510.1327924214553373No Hit
CGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGCGGCGGCGGG282540.12443756677387482No Hit
GGGGTCCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGG273850.12061027699095923No Hit
CCTCTCTCTCTCTCTCTCCCCCGCTCCCCGTCCTCCCCCCTCCCCGGGGGA270360.11907319513337862No Hit
CGCCGCCCGCCGCCGCCGCCGCGCGCCGAGGAGGAGGGGGGAACGGGGGGC261080.11498605483585772No Hit
CCGCGCGCCCTCCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGA260130.11456765146488304No Hit
CCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACC243180.10710245447749303No Hit
CCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCC242230.10668405110651835No Hit
CCCACGTCTCGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTCGGGCGG240790.10604983968104098No Hit
GTCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCGCCCCCCGG228500.10063702133443193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG62800.042.56489641
TCGTATG62750.042.56192445
CTCGTAT63000.042.39368444
TCTCGTA63150.042.32861743
ATCTCGT63150.042.29326642
GTCACAT65000.041.43286529
CACATTC65250.041.30896831
CTTTATC64950.041.19181438
ACATTCC65500.041.1876432
CCTTTAT65550.040.9174837
TCACATT66150.040.78059430
TCCTTTA65800.040.76193236
AGTCACA66350.040.6236728
CATTCCT66600.040.5084433
TTATCTC65900.040.4944940
CCAGTCA67300.040.0836626
CAGTCAC67300.040.0502327
ATCGGGG3500.039.864591
ATTCCTT68400.039.4096234
GCCCTGT33400.039.2812921
Produced by FastQC (version 0.11.5)