FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24849609
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6301172.5357219906357478No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC484420.19494069303062272No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA415300.1671253660369465No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA371410.1494631163009446No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT354850.14279902754204302No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC286850.11543441186539394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG43850.020.678556
GCAGACG43600.019.5079612
CGAGACG55550.019.19816642
CAGACGT46550.018.5133743
CCGCGAC35950.017.89915745
AGACGTT51250.017.7364944
GACGTTC52000.017.5238425
GTCGTCG51850.017.48754118
TCCGGAT82650.017.4779287
CGGGCGA62750.017.2821938
GGCGAGA63800.017.13882440
GTTCGTC11050.017.1197281
GCGGGCG66900.016.84908137
CGACACG60950.016.79589541
ACACGGC62050.016.7519643
GGGTCGT54300.016.65680316
AACGCCG11150.016.54640230
TGGGTCG55800.016.28968215
CGTTCGA56500.016.089067
TGCGCGT6750.015.99937245
Produced by FastQC (version 0.11.5)