FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39143673
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCA442550.11305786250564683No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC403430.1030639102263091No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC393010.10040192191468594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG44000.028.2775276
TATTGCG81250.027.05540542
CGTTCGA47200.026.408317
GTCGTCG53300.023.42839218
GACGTTC56600.022.499415
TGGGTCG57450.021.85399615
TCGGCGC70750.021.59384745
GCAGACG58250.021.554932
GCGGTAC103750.021.36162429
GGGTCGT59450.021.34587516
CAGACGT62050.020.9947513
CCTTGCG106700.020.98173725
GTTCGAA62400.020.7687688
AGACGTT62250.020.6020574
TTGCGCC108750.020.31738744
GCGTGCG60350.020.2812937
CTTGCGG110900.020.16690826
CGAATGG65250.019.65541311
TGCGCCA112200.019.65259645
GCGATCG62450.019.59969341
Produced by FastQC (version 0.11.5)