FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43390444
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCA516580.1190538635649822No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA502830.11588496305776452No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG458800.10573756746992495No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA444760.10250183197019141No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGA435090.10027323066802452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG41900.026.0968656
TATTGCG107350.024.81820342
CGTTCGA45100.024.1954277
GTCGTCG49900.021.59663418
GCAGACG56650.020.338072
CCTTGCG133250.020.17821725
GCGGTAC131600.020.10675629
GACGTTC56700.019.9587125
GGGTCGT55500.019.7418616
GTCGGAT21350.019.6150781
CTTGCGG135950.019.59562326
TACGCCG120700.019.59074821
TTGCGCC137100.019.4654944
CAGACGT58900.019.4068053
TTGCGGT136650.019.36367427
TGCGCCA140050.019.08793645
AGACGTT60200.018.949234
TGGGTCG58700.018.74229815
GTTCGAA60100.018.6059368
GGTACTA147400.018.40837331
Produced by FastQC (version 0.11.5)