FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35043732
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG1348510.3848077596301673No Hit
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCA1315430.3753681257464245No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGC1195760.3412193655630057No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA1164060.3321735253539777No Hit
GTTAGATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCT1122640.3203540079578282No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGA1112170.3173663124692313No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAG1063860.3035806802768609No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT1037380.2960244074461019No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC966680.2758496155603518No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA926400.264355405982445No Hit
GTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTGTGTCAACTGGAGT872200.2488890167291543No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT847430.24182070562575927No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAGATTTTTTACTCTCTCTACAAGG826170.2357540001732692No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC810830.23137661251375852No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT741140.21149003194066204No Hit
GGGTGTTAGATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAG730920.20857367588589024No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACC700660.1999387508156951No Hit
AGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCA694860.1982836759509518No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAG681340.19442563936968815No Hit
GTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGC633230.18069707872437787No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACT611250.17442491570247143No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCAT596220.1701359889409039No Hit
GTGAGGTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGAT591880.16889753636969942No Hit
GGCTGCACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATT551850.15747466622561776No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC549930.15692677937384067No Hit
GGCTGCTTTTAGGCCTACTATGGGTGTTAGATTTTTTACTCTCTCTACAAG526560.1502579690998664No Hit
GTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATGG525270.1498898576213287No Hit
GGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCGCCA510240.14560093085976117No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG503420.1436547911050113No Hit
GGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTT500660.14286720375558173No Hit
GGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT498630.14228792755292158No Hit
CTCCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGT495710.14145468296584393No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATTA494160.14101237847612805No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAAC479500.13682903407662175No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACG471650.1345889758545123No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGA467810.13349320215095814No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTA465730.13289965806153295No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGG465190.13274556488447065No Hit
GAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTC462510.13198080615386512No Hit
GCCCTGTTCAACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTC459340.13107622213296233No Hit
CCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAA450320.12850229536055122No Hit
GGAGAATGTTTTCATGTTACTTATACTAACATTAGTTCTTCTATAGGGTGA426780.12178497427157586No Hit
CTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAAATTG423310.1207947829300829No Hit
GTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGT420230.11991588110535716No Hit
GAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTT402010.11471666316818083No Hit
AGATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTT401330.11452261990817644No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAA400630.11432286949346604No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGAT391670.11176606418517297No Hit
CGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT388120.11075304422485596No Hit
CTCGTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTC387470.1105675616969106No Hit
GTTTAGGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTA385500.11000540695836848No Hit
GCCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGA377170.10762837702331476No Hit
GTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGCTGAAGATGG368520.10516003261296485No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCC367110.10475767820619106No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTT358090.10218375143377995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG63250.029.453186
GTCGTCG69800.026.6566218
GCAGACG71600.026.2755432
GACGTTC71600.026.2687975
CAGACGT71700.026.1110063
TATTGCG248950.026.05809242
CGTTCGA71900.025.9723727
TGGGTCG74300.025.31464415
AGACGTT73950.025.1629734
TCGGCTT25950.024.1082612
GGGTCGT77850.024.1025516
TGCGCCA270050.024.03072245
GGTACTA275300.023.95383831
GGTCGTC81150.023.67690517
CTATTGC282100.023.45826341
CGAATGG81000.023.41601811
GCTATCA150950.023.28402343
GCGGTAC283300.022.96879829
GTACTAT289350.022.9617132
TCGAATG84200.022.71285410
Produced by FastQC (version 0.11.5)