FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56207701
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCC784530.1395769593920947No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA654470.11643778136380278No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGA637150.11335635307339825No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCG599450.10664908710640914No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG583520.10381495589011905No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG564560.10044175263457228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA155850.028.4766967
ACGTTCG157200.028.3179996
GCAGACG163950.026.7403682
CAGACGT166450.026.3657533
GACGTTC171200.026.0285535
GTCGTCG171850.025.73394818
AGACGTT174050.025.2918874
GGGTCGT175300.025.22762516
TGGGTCG183100.024.22673415
TATTGCG116100.024.10711342
GTTCGAA188050.023.9714168
TCGAATG188100.023.96517410
CGAATGG190600.023.532911
GCGTGCG175950.023.4639537
TTCGAAT196900.022.9854569
CGTGCGA187600.022.10297438
GCGATCG193300.021.98676941
GGGGCGT185600.021.74610134
GGCGTGC192300.021.62104236
TGCGATC199300.021.33613840
Produced by FastQC (version 0.11.5)