FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51956069
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10217691.966601822782243No Hit
GTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTT1043160.20077731438843072No Hit
CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTA1019560.1962350153934856No Hit
CCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCT832970.16032198278895965No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA769720.14814823654191392No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA761070.14648336847808868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCG11700.016.7321533
GTTCGTC18200.014.9805891
TACGGGC10550.014.0771562
CGGGCTA12400.012.8827664
TACGCGA1801.6956892E-512.5006979
CTACGGG12600.012.1605521
GTCCCGT28450.011.3257941
CCGCGTC71950.011.22583242
CTCGATT160800.011.1402781
CGTCGCG2452.900897E-611.0213193
ACCGCCG83800.010.6053634
ACGCGGC16550.010.6039535
CGCGTCC76900.010.53249143
CTACGCG2605.9079484E-610.3851958
GGGGGCC199800.010.364181
CGTATAG6550.010.3203611
CGGGAAT25000.010.2749511
TCGGGGG89350.010.1492492
ACGGGCT16700.09.8361433
CGATTGG63250.09.81899453
Produced by FastQC (version 0.11.5)