FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005331590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005331590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69139971
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCC1338780.19363328920111927No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG1252610.18117016566292746No Hit
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCC1217970.17616003917618073No Hit
CTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTG1190250.17215078091369174No Hit
CTCCTCTAGAGGGGTATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGT1127400.16306052543759383No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA1118920.16183402796046878No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAGA1047160.15145508232857083No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACT966310.13976141239631124No Hit
CTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCA949790.13737205646210063No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCAT919680.13301712261348791No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC882550.12764685712697219No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCC860310.12443019393224795No Hit
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTT857610.1240396817638237No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT838580.12128729414711498No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGC832350.12038622347701014No Hit
GTTCAGATATGTTAAAGCCACTTTCGTAGTCTATTTTGTGTCAACTGGAGT814290.11777413097266123No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAG797290.11531535065295298No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGG762810.11032836562803881No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGC760730.11002752662421568No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAAC760100.10993640711825002No Hit
GTGAGGTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGGT740670.10712616584695994No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCACG734870.10628728785553004No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGAC728310.10533848792039556No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT712720.10308364173308662No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGCC707890.10238505885401658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT48950.029.1857666
CCGCGTT52150.027.3949667
AGACCGC57150.025.5503084
ACGTTCG134400.025.1766916
CAGACCG61600.024.3620263
GAACGAT63050.023.1985245
GTCGTCG155250.021.60758218
GCAGACG158950.021.4461632
GACGTTC161200.021.2282225
CAGACGT162100.021.1251583
GGGTCGT160050.021.10016316
TGGGTCG161500.020.9942915
CGTTCGA162000.020.8873887
ATCGACG19700.020.67342441
CGCGTTC69950.020.58478
AGACGTT165800.020.4908564
TATTGCG228900.020.25976642
ATACGGA72800.020.02749833
TCGGCGC162050.019.4823245
TCGAATG177300.019.33896410
Produced by FastQC (version 0.11.5)